PCR Sensitivity Kit for PTC

The PTC (PhenylThioCarbamide) is a bitter organic compound found in certain plants like Brussels sprouts or broccoli, about 30% of the population is sensitive to this compound. The objective of this lab practical is to invite students to establish the relationship between phenotype and genotype, based on their own genetic heritage. The phenotype is easily characterized using test strips, and the genotype is determined by gene amplification of the TAS2R38 gene from a simple collection of cells from the epidermis (buccal or the back of the hand). The TAS2R38 gene, located on chromosome 7, encodes the receptor for PTC. Allelic variation between individuals is based on the mutation of 3 nucleotides (SNP = Single Nucleotide Polymorphism). Amplification will identify one of the 3 SNPs, as this mutation corresponds to a restriction site. PCR is followed by enzymatic digestion, if the mutation is present, 2 fragments will be visible (T-), while a single fragment corresponds to the non-mutated allele (T+). Amplification duration: 1 hour 15 minutes Enzymatic digestion duration: 20 minutes Electrophoresis duration: 15 to 25 minutes depending on the system Experiment files and educational videos available on the Jeulin digital platform!

For 18 tests: PCR microtubes, Sampling swabs, Primers, Ready-to-use PCR reagents, Restriction enzyme, Molecular weight ladder.
Storage: -20°C Use-by date of 6 months