PCR Kit Searching for the AMEL gene in birds

Tracing back in time to the archosaurs, by finding traces of the AMEL gene in the genome of birds.
Developed in collaboration with the National Museum of Natural History, this PCR kit uses amelogenin, a protein present in the dental enamel of vertebrates.

Interest: To show that mammals and birds had a common ancestor. To identify evolution mechanisms and selection pressure.

Following the history of amelogenin is to look at over 600 million years of vertebrate evolution. PCR amplification, sequence comparisons, students have the opportunity with the AMEL gene to manipulate tools of evolutionary biology as we can do at the Institute of Systematics, Evolution, Biodiversity. 

The activities were designed in collaboration with Sidney Delgado, Associate Professor at Sorbonne University Homology Team - ISYEB National Museum of Natural History.

Conservation: - 20°C Shelf life of 12 months

For 18 amplifications:
- Chicken and Human XY and XX blood samples
- 2 tubes of ready-to-use H + G primers*
- 2 tubes of PCR Mix [Nucleotides + Taq polymerase]
- 1 tube of DNA release
- Molecular weight marker
- Sampling loops
- 0.2 ml PCR microtubes

* Primers work equally well on the provided synthetic gene (plasmid) and on cells taken from the skin, so the experimenter has the choice of protocol!