PCR Kit Alu Sequence locus PV92

This manipulation aims to study a mutation in the human genome caused by the insertion of an Alu transposon.
Alu sequences are 300 bp repeated elements, specific to primates and dispersed throughout the genome. The exploitation of the results will allow us to address the geographical distribution of allele frequency, thus inferring ancestral migrations of Homo sapiens populations.
With a million copies, Alu sequences represent 10% of the total genome, the PV92 locus is a particular insertion located on chromosome 16. This locus reflects the recent evolutionary history of the Homo genus and is not found in all individuals. The PV92 site has two alleles, the + alleles (presence of the insertion) and - alleles (absence of the insertion) which generate DNA fragments of different sizes, identifiable by electrophoresis after PCR amplification. This results in three possible PV92 genotypes (++, + -, or --).

This complete and easy-to-use kit contains the material for a direct sampling of epidermal cells (buccal or above the hand). No need for centrifugation or extraction, you simply and quickly obtain your DNA samples.

Amplification time: 1 hour 10 minutes
Electrophoresis time: 15 to 25 minutes depending on the system

For 18 tests: PCR microtubes, Sampling swabs, Primers, Ready-to-use PCR reagents, Molecular weight ladder.
Storage: -20°C 6-month shelf life