Cystic Fibrosis Kit (DNA electrophoresis)

Cystic fibrosis is a severe genetic condition characterized by an increase in the viscosity of bodily secretions, particularly affecting the respiratory and digestive systems. Over 1500 identified mutations in the CFTR gene alter the function of the CFTR transmembrane protein synthesis. The activity is presented in the form of a pre-implantation diagnosis (fictional). Parents wishing to have a child through IVF undergo this diagnosis due to family history. This lab practical follows the principle and format of the CF4 medical test (Zeneca diagnostic), a test based on PCR ARMS amplification. It identifies the 4 most common mutations of the CFTR gene: DeltaF508 / G542X / G551D /621+ through electrophoresis. The principle involves a dual analysis where a healthy gene is sought in one tube and its mutated allele in the other tube. Each individual has 2 tubes containing the PCR products, determining their genotype based on the presence or absence of one or more mutations. The DNA solutions are calibrated and require no preparation. Format: 20 tests, totaling 80 samples to analyze + 10 samples (molecular weight) Experiment duration: 60 minutes Storage duration: 6 months Storage condition: -20°C DNA fragment separation: 2% agarose gel The kit includes: - 4 calibrated DNA tubes ready for deposition (parents 1 and 2 (A + B)) - 1 tube of molecular weight ladder Cystic fibrosis: from gene to cellular phenotype We offer a comprehensive approach to studying this genetic disease. 1 - Observe tissues of affected individuals under a microscope (cellular phenotype): ref. 575251 2 - Visualize DNA mutations directly (DNA electrophoresis): ref. 117227 3 - Visualize CFTR proteins in a healthy individual and a carrier of the disease (protein electrophoresis): ref. 117275 Also check out: PCR | PhorEasy® Electrophoresis System PCR | Choosing a thermocycler for teaching PCR | Performing electrophoresis Brochure | Our educational kits for teaching The educational software PhorEasy® is available for free on the Jeulin digital platform.

The kit consists of:
- 4 calibrated DNA tubes ready to be deposited (parents 1 and 2 (A + B))
- 1 tube of molecular weight ladder