DNA footprint analysis kit - 1660007EDU | Bio-Rad

The genetic fingerprint analysis kit allows your students to use real DNA as evidence while playing the role of investigators at the crime scene to discover for themselves "Who is the perpetrator of the crime?".
 
Principle:
The six DNA samples contained in the genetic fingerprint collection kit are plasmids designed to mimic the natural variations of DNA that exist between one human being and another. One DNA sample was taken from a "crime scene" and five samples were obtained from various "suspects". Each DNA sample is digested using a mixture of two DNA restriction enzymes, which generates a distinct set of DNA fragments for each sample. The resulting DNA fragments are separated by electrophoresis on agarose gel and visualized to identify the culprit. 

Lab Duration: 2 sessions of 45 minutes
Format for 32 students: 8 stations (4 students per station)
Shelf Life: 12 months

The kit consists of:
- 100 µl standard size DNA (HindIII lambda DNA digestion)
- 60 µg DNA from the crime scene
- 60 µg DNA from the suspect
- EcoRl/Pstl restriction enzyme, lyophilized, 3,000 units
- 1 ml sample loading buffer
- 100 ml Electrophoresis buffer, 50x TAE
- 2.5 ml Sterile water
- 5 g Agarose powder
- 100 ml Fast Blast™ DNA dye, 500x
- 60 Colored microcentrifuge tubes, 2.0 ml
- 30 Microcentrifuge tubes, 1.5 ml
- 8 Foam floats
- 1 Instruction manual