Bio-Rad | CRISPR-Cas9 Gene Editing Kit

1 kit 4 activities!

- Model the components of the CRISPR-Cas9 system

- Use CRISPR-Cas9 to modify the lacZ gene in E. coli.

- Use PCR and gel electrophoresis to confirm editing in the transformants (optional).

- Use bioinformatics to design Cas9 target sites in the human genome and determine the risk of off-target effects.

Principle:

Carry out a real CRISPR-Cas9 gene editing with your students. Thanks to this innovative technology, deactivate the lacZ gene in E. coli to produce a phenotype that can be easily visualized through blue-white screening.

Duration of the lab: 2/3 sessions of 45 minutes
Format for 32 students: 8 stations of 4 students
Shelf life: 12 months

The kit consists of:
- E. coli HB101-pBRKan, lyophilized
- Donor model DNA (pLZDonor)
- Donor model DNA and guide RNA (pLZDonorGuide)
- KIX Mix
- Spectinomycin powder
- Arabinose, lyophilized
- Sterile transformation solution
- LB nutrient agar powder
- 50 ml LB broth capsule
- 80 Sterile inoculation loops
- 60 Sterile Petri dishes
- 90 Sterile 2.0 ml microcentrifuge tubes
- 1 Instruction manual

Technical characteristics

Thématiques

Biotechnologie

Documentation

CRISPR-Cas9 Gene Editing Kit + extension - 12012608EDU | Bio-Rad