The medium consists of a basal medium of MURASHIGE and SKOOG (agar-agar and mineral salts) enriched with auxin (IAA, 0.2 mg/L) and cytokinin (BAP, 0.2 mg/L).
SP1 medium in 190 mL agar bottle - Propagation
Objective:
Implement a protocol to obtain the development of a whole plant from the sampling of a Saintpaulia leaf fragment. It is necessary to work with 2 successive mediums, the multiplication medium (SP1) and then the rooting medium (SP2).
The pre-sterilized and ready-poured SP1 medium in a flask helps reduce the risks of contamination during material handling.
Why choose Saintpaulia?
This indoor plant, available in garden centers, is easy to maintain. It allows for easily obtaining disinfected fragments, unlike the potato, which requires the prior production of sprouts. It is also simpler to cultivate in vitro than carrot tissues. It is not necessary to obtain a sterile callus beforehand.
Capacité | 190 ml |
Format des milieux | Gélose |
Vendu par | À l'unité |
Stérile | Oui |
Caractéristiques techniques
The medium consists of a basal medium of MURASHIGE and SKOOG (agar-agar and mineral salts) enriched with auxin (IAA, 0.2 mg/L) and cytokinin (BAP, 0.2 mg/L). |