SP1 powder for 1000 mL - Multiplication
Dosage for the preparation of 1000 mL of SP1 culture medium.
Objective:
Implement a protocol to achieve the development of a whole plant from the sampling of a Saintpaulia leaf fragment. It is necessary to work with 2 media successively, the multiplication medium (SP1) and then the rooting medium (SP2).
Why choose Saintpaulia?
This indoor plant, available in garden centers, is easy to maintain. It allows for easily obtaining disinfected fragments, unlike the potato which requires the prior production of sprouts. It is also easier to cultivate in vitro than carrot tissues. It is not necessary to obtain a sterile callus beforehand.
Capacité | 1000 mL |
Format des milieux | Poudre |
Vendu par | À l'unité |
Caractéristiques techniques
Storage: 4 months at 4°C (see product label).
The medium consists of a basal medium of MURASHIGE and SKOOG (agar-agar and mineral salts) enriched with auxin (IAA, 0.2 mg/L) and cytokinin (BAP, 0.2 mg/L). |