Bacterial Transgenesis pGLO Kit - 1660003EDU | Bio-Rad

Use Bio-Rad's Bacterial Transgenesis pGLO Kit (Cat. No. 115057) to teach gene expression and genetic engineering. In this lab activity, students use the pGLO plasmid to transform bacteria to express green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea Victoria, which causes bacteria to glow green under UV light. Explore the concepts of DNA > RNA > Protein > Trait and the roles external and internal factors play in gene regulation. Features and Benefits: Hands-on genetic engineering — from manipulating plasmids to performing heat shock and seeding transformants, students complete a comprehensive transformation protocol — the same protocol used daily by scientists around the world. Visible and spectacular phenotype — bacteria transformed with the pGLO plasmid glow fluorescent green under UV light. Once students have completed this lab activity, they never forget the central dogma of molecular biology: DNA > RNA > Protein > Trait — Green Fluorescence. Gene expression and regulation — Bacteria transformed with the pGLO plasmid show green fluorescence only when cultured in the presence of arabinose. Use this experiment to illustrate that not all genes are expressed at the same time and that environmental factors control when gene expression takes place. Duration Manipulation: Complete all activities in two 45-minute sessions.

Kit contains enough material for 8 student workstations (2-4 students per workstation) Plasmid (pGLO), freeze-dried 20 μg E. coli strain HB101 K-12, lyophilized LB Nutrient Broth, sterile 10 ml Powder of LB nutrient agar 20 g Ampicillin, lyophilized 20 mg Arabinose, lyophilized 600 mg Sterile transformation solution (CaCl2) 100 ml Petri dishes, 60 mm, sterile 40 Inoculation loops, sterile 80 Microcentrifuge tubes, 2.0 ml, sterile, color coded 60 Disposable plastic transfer pipettes 50 Foam micro test tube holders 8 UV penlight 1