PCR Kit Transformed Yeast GFP

Objective of the practical work: Verify the presence of the GFP gene responsible for the fluorescence of a transformed yeast through PCR amplification.

The student has 2 strains of S. cerevisiae:
- 1 wild-type untransformed strain (control)
- 1 strain transformed with GFP
The complete procedure is carried out in 3 steps: sample extraction/purification, PCR amplification, and agarose gel electrophoresis.

The student can also test the strains obtained through the practical work Yeast Transgenesis ref. 117115. The protocol and primers are compatible.

Duration of the practical work: 2 sessions of 60 minutes
Format for 20 students
Storage: 12 months at -20 °C

Composition: PCR microtubes, primers, ready-to-use PCR reagents, 2 yeast strains, molecular weight ladder