Yeast Transgenesis Kit: Expression of a GFP Fluorescent Protein

This kit offers a new, simplified protocol for performing inter-species gene transfer. Through transgenesis, the gene encoding the fluorescent protein from the bioluminescent tissues of the jellyfish will be expressed by the yeast S. cerevisiae. An ultra-simple transformation protocol adapted for the school environment! Jeulin has designed a protocol that does not require making the cells competent. Simply resuspend fresh yeast cells from a colony, mix them with the transformation solution, plasmid DNA, vortex, and then let it incubate for 30 minutes at 42°C. The yeast cells are then cultured to verify the efficiency of the transfer, and the expression of the bioluminescence gene is revealed by illuminating the boxes with UV radiation. Format: 15 tests Duration of the experiment: 120 minutes + 1 session to read the results Storage duration: 1 month Storage condition: +4°C (DNA Microtube at -20°C for long-term storage)

The kit consists of: - Yeast strain on plate (x1) - Transformation solution (1.9 ml) - 1 microtube of plasmid DNA (80 µl) - 1 microtube of DNA carrier (78 µl) - 1 ready-to-use YPD medium (200 ml) - 1 ready-to-use selective URA- medium (200 ml) - 30 Petri dishes of 55 mm - 15 inoculation loops - 15 sterile sticks - 15 sterile microtubes for transformation - 1 sterile 90 mm Petri dish (for pre-culture before the practical work)