Antibiogram Kit 2 strains

! * NEW COMPOSITION * !

Objectives: Measure the effectiveness of 4 antibiotics on 2 strains of bacteria.

Carrying out an antibiogram protocol illustrates the concept of bacterial genetic variation, a mechanism behind the emergence of bacterial strains resistant to antibiotics. Antibiogram using the diffusion method in a gel medium.
After a quick reactivation of lyophilized strains, each pair seeds the two bacterial strains on two separate gel plates. 4 different antibiotic discs are then placed. The results are read after 24 to 48 hours. Inhibition of bacterial growth is indicated by a 'clear' circular zone without bacteria, the zone being wider the more sensitive the bacteria are to the antibiotic.

Format: 25 tests
Experiment Duration: 60 min (incubation 24h to 48h)
Storage Duration: 4 months 
Storage Conditions: + 4°C 

The kit consists of:
- 1 tube Inoculum Escherichia coli, lyophilized dense culture (Pasteur Strain).
- 1 tube Inoculum Staphylococcus epidermidis, lyophilized dense culture.
- 2 sterile tubes of physiological water (2 x 50 ml)
- Antibiotics (4 x 50 discs) ampicillin 10 µg, penicillin 10 µg, tetracycline 30 µg, nalidixic acid 30 µg
- Mueller-Hinton medium in ready-to-pour flask (5 x 200 ml)