Comparative Proteomics Kit II: Western Blot Module - 1662800EDU | Bio-Rad

This kit is the follow-up to the Comparative Proteomic Kit I: protein profiling ref. 117179.
With your students, apply the Western Blot technique to the results obtained after the polyacrylamide gel electrophoresis (kit I) to specifically identify the myosin light chain among the hundreds of proteins that make up the muscle cell extracts of fish species.
 
Protocol:
Students will transfer protein bands from polyacrylamide gels to a membrane, then use an anti-myosin light chain antibody to precisely identify which protein in the profile of each species is the myosin light chain. By using bioinformatics databases, students can compare their experimental results with real protein sequence data derived from DNA and RNA sequences and determine if variations in myosin between species are due to "genetic" or "epigenetic" factors.

Lab Duration: 4 sessions of 45 min
Format for 32 students (8 stations of 4 students)
Storage Duration: 12 months

The kit consists of:
- Lyophilized primary antibody
- Lyophilized secondary antibody
- HRP Color Detection Reagent A
- HRP Color Detection Reagent B
- 1 L Tris-glycine 10x
- Non-fat dry milk blocker 10x
- 2 x 100 ml Phosphate buffered saline (PBS)
- 5 ml 10% Tween 20
- 8 sheets of 0.45 µm nitrocellulose
- 16 sheets of blotting paper
- 25 reagent tubes
- Instructions (guides + manual)