qPCR : Real-Time PCR Detection SystemBio-Rad Innovates for Education

Cutting-Edge Products

What is real-time PCR?

Quantitative Polymerase Chain Reaction (qPCR) is a molecular biology technique used to amplify and quantify DNA or RNA in a sample. Unlike conventional PCR, real-time qPCR monitors the amplification process in real-time using fluorophores, enabling fast and precise nucleic acid quantification. This technique is widely applied in:
  • Gene expression analysis
  • Molecular diagnostics (genetic diseases, viral or bacterial infections)
  • Mutation and antibiotic resistance detection
  • Protein studies (FRET and thermal analysis)
In the laboratory, qPCR relies on a thermocycler equipped with an optical detection system that measures fluorescence signals, which are directly proportional to the amplified DNA quantity, ensuring accurate quantification from the first cycles.

CFX Duet – A benchmark in functional genomics
Prepare your students to become true professionals

Bio-Rad real-time PCR detection systems (708290)

Key benefits:
  • Installation, setup, and commissioning
    Handled by Jeulin engineers trained by Bio-Rad
  • 2-year warranty covering parts and labor
    If a part or repair is needed, after-sales service is free during the warranty period and fast (workshop in Germany).
  • No maintenance or calibration required throughout the device's lifespan, thanks to the Diode/Photodiode system
  • Estimated lifespan: 25 years
    For one PCR run per day; LED/Photodiode technology
  • Compatible with all market-standard consumables, including:
    Microtubes with flat caps, 96-well low-profile plates with/without skirt of optical quality.

Why choose this system?

1. No recalibration needed thanks to the Diode/Photodiode pair:
Before starting measurements, the device automatically aligns the diodes.

2. Fast detection:
With its filtered LEDs and high-sensitivity photodiodes, the CFX Duet enables precise analyses in a short time. It can scan all channels in just 12 seconds.

3. Reads all 96 wells in every run:
The system reads all 96 wells in each run—even empty wells! If a sample is misplaced on the plate, you will still get results. You can also create or modify the plate layout afterward.

4. Application flexibility:
- Multiplexing up to 2 simultaneous targets for optimized analyses.
- Ideal for advanced experiments such as FRET or thermal protein analysis—features rarely available at this price.

5. Unmatched precision and thermal uniformity:
The CFX Duet provides a thermal uniformity of ±0.3 °C, ensuring reproducible results, even for demanding applications like diagnostics or quantitative analysis.

6. Cost-effective and compact:
With optimized dimensions and a competitive price, the CFX Duet is an economical solution for laboratories needing high-performance equipment without compromising quality.

Model CFX Duet
Capacity 96 wells | 1 à 50 µL
Detection 2 targets per well
Excitation / Emission 3 LED / 3 photodiodes
(450-580 nm)
Screen No
Software CFX Maestro
(multi-user | free lifetime support)
Scan speed (96 wells) 12s for all channels,
3s for a single channel
Temperature range 4 - 100°C
Temperature gradient Yes
FRET Yes
Built-in memory Yes + data retrieval via USB (and more for Opus)

Technical documentation and guides

Ready to optimise your PCR analyses?
Throughout the world, a network of distributors at your service

Logiciel CFX Manager

Optimized for the CFX Real-Time PCR series, the CFX Maestro Software 2.3 is a powerful and intuitive tool designed to collect, analyze, and interpret real-time PCR (qPCR) data. Ideal for education, it supports users through all essential steps, from plate setup to statistical analysis and result visualization. This software ensures a smooth experience while offering advanced features such as reference gene selection and powerful graphical tools.

  • Quick setup with a clear interface
  • Lifetime free updates
  • A single license purchase allows installation of the software on all computers in your institution!

Educational Support:

qPCR Starter Kits

Comes with all the reagents needed for qPCR.

Investigation on a Crime Scene

RT-PCR Starter Kit: Crime scene investigation

Uncover the murderer by amplifying and quantifying the DNA of the suspects!
 - Perform real-time quantitative PCR (qPCR) in clas
 - Complete kit for 32 students

Investigation on GMOs

RT-PCR Starter Kit: GMO investigation

Test and quantify the presence of GMOs in food.
 - Perform real-time quantitative PCR (qPCR) in class
 - Complete kit for 32 students

A complete Bio-Rad range to discover on our website