Crime Scene Investigation PCR Kit - 1662600EDU | Bio-Rad

This introductory PCR kit allows students to simulate DNA profiling as commonly used in forensic science labs. The kit is designed to introduce students to the concepts of PCR in two lesson periods, without having to go through the complex steps of genomic DNA extraction. With this kit, students explore the use of PCR and STR alleles to solve crimes. They become crime scene investigators by performing forensic DNA analysis in the real world. Using PCR and agarose gel electrophoresis, they analyze provided DNA samples — from a crime scene and hypothetical suspects — to learn how DNA profiling determines a genotype ("barcode") and can distinguish one person from another. This powerful tool aids in crime scene investigations, missing persons, mass disasters, immigration litigation, and even paternity testing. Features and Benefits: Teach PCR in the context of genetic profiling—use PCR to amplify multiple DNA samples, then analyze the results using agarose gel electrophoresis to find the culprit. Simulate the analysis of a crime scene like real forensic scientists do! No DNA Extraction Required — The plasmids used in this kit were designed to mimic the natural variations in human DNA that exist at a single STR locus — no complex genomic DNA extraction is required. Explore the Power of Discrimination—Extension exercises allow students to see for themselves how using more loci allows finer discrimination between two people. Duration Manipulation: Complete all activities in two 45-minute sessions

Kit contents support 32 students (8 x 4-student workstations) Crime scene DNA sample, 250 µl Suspect A DNA sample, 250 µl Suspect B DNA sample, 250 µl Suspect C DNA sample, 250 µl Suspect D DNA sample, 250 µl PCR master mix (Taq DNA polymerase, dNTPs, buffer), 1.2 ml Primers (blue), 25 µl Allele ladder , 200 µl Orange G Loading Dye, 1 mL PCR Tubes, 0.2 mL (50) Capless PCR Tube Adapters, 1.5 mL (50) Colored Micro Test Tubes, 2.0 mL (60) teaching, including teacher's guide, student's manual and quick graphic guide.