Complete package In vitro effects and detection of bacteriophage T4 by PCR
Sku: 117236
Jeulin
The strong points
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Designed by Jeulin
€162.75
€195.30
Our complete pack allows you to introduce your students to bacteriophages, essential actors in the balance of microbiomes, in 3 complementary steps:
1 - Bacteriophages antibacterial effect Kit (117234)
2 - Bacteriophage virus detection PCR Kit (117235)
3 - Bacteriophage virus titration
Principle of the experiment for kit 1:
Compare in vitro the bactericidal effect of bacteriophages to that of two antibiotics. This activity follows the principle of an antibiogram. A culture plate of E. coli is divided into 4 zones: 2 antibiotics are tested in comparison to a drop of a solution (bacteriophages), the 4th quarter is the control zone.
Compare in vitro the bactericidal effect of bacteriophages to that of two antibiotics. This activity follows the principle of an antibiogram. A culture plate of E. coli is divided into 4 zones: 2 antibiotics are tested in comparison to a drop of a solution (bacteriophages), the 4th quarter is the control zone.
Principle of the experiment for kit 2:
The results of TP1 show that an element, not visible under the microscope and present in the deposited suspension, produces zones of inhibition of bacterial growth equivalent to those of certain antibiotics. PCR analysis will determine the presence of a viral gene in the suspension and therefore that this bactericidal effect is due to a virus: bacteriophage T4.
Principle of experiment 3:
From the bacteriophage solutions of TP1 and/or TP2 (quantities are sufficient to carry out this additional activity), it is possible to determine the titer of the bacteriophage suspension. This allows visualizing that each micro-lysis plaque is the result of the action of a single virus.
The activities were designed in collaboration with Laurent Debarbieux, Research Director of the "BACTERIOPHAGE, BACTERIA, HOST" unit at the Pasteur Institute in Paris.
Composition of kit 1 for 40 boxes: - Mueller Hinton medium 1L- 1 tube of Escherichia coli inoculum (k12 type), lyophilized dense culture.(Pasteur strain)- 1 tube of bacteriophages T4
- Penicillin-impregnated discs (x 50)
- Tetracycline-impregnated discs (x 50)
- 40 Petri dishes
Composition of kit 2 for 20 amplifications:
- 2 tubes of ready-to-use primers
- 2 tubes of PCR Mix [Nucleotides + Taq polymerase]
- 1 tube of bacteriophages T4
- 1 tube of DNA release
- 1 tube of molecular weight marker
- 1 extraction buffer
- 2 x 10 PCR microtubes 0.2 mL
For the titration of a bacteriophage virus (experiment 3) it is necessary to add the PBS buffer (ref. 107843) in addition to the pack.
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