Beer-Lambert Law Experiment

Topics covered:
- Comparison method for measuring concentration
- Color scale
- Spectrophotometric analysis
- Absorbance and absorption spectrum

Principles and objectives:
The protocol first uses a color scale to provide an estimate of the concentration value of a solution containing a single dissolved colored chemical species. Then, it utilizes a spectrophotometer and relies on the Beer-Lambert law A = k x c to determine an accurate value of the concentration of one of the colored chemical species dissolved in the studied solution. The protocol may also involve studying the absorbance spectrum A = f( λ ) to identify the nature of the colored chemical species dissolved in the solution.

Composition:
▪ 1 Spectrophotometer Spectrovio II (ref.204603) with embedded software
▪ 2 burettes 25 ml (ref.713318) on stand
▪ 1 set of 10 test tubes (ref.181011) on stand
▪ 1 set of 15 stoppers no. 1-solid (ref.703500)
▪ 2 beakers 100 mL (ref.713119)
▪ 1 set of 100 cuvettes for spectrophotometer (ref.701119)
▪ 1 stock solution of 1.0 L of potassium permanganate (ref.104050)
▪ 1 wash bottle 250 mL (ref.723186)
▪ 1 solution of 5 L of distilled water (ref.107625)