Mutation Kit ΔF508 Cystic Fibrosis CFTR (protein electrophoresis)

Visualize the consequences of a deletion mutation on cystic fibrosis.

Scientific observation: The ΔF508 mutation affects the production process of the CFTR protein, preventing it from reaching its normal final destination, which is the cell membrane. The CFTR remains trapped inside the endoplasmic reticulum.

Lab experiment principle: a comparison is made between the purified endoplasmic reticulum fraction and the cytosolic fraction of healthy individuals and individuals carrying the ΔF508 mutation.
For a healthy individual, the electrophoretic profiles are identical for both fractions, whereas a carrier of the CFTR mutation will show different cytosolic and endoplasmic reticulum fractions. 

Lab duration: 60 min
Format for 40 students, with over 90 deposits of protein fractions to analyze 

Cystic fibrosis: from gene to cellular phenotype
We offer a comprehensive approach to studying this genetic disease.
1 - Observe tissues from affected individuals under a microscope (cellular phenotype): ref. 575251
2 - Visualize DNA mutations directly (DNA electrophoresis): ref. 117227
3 - Visualize CFTR proteins from a healthy individual and a disease carrier (protein electrophoresis): ref. 117275

Also consult:
PCR │ PhorEasy® electrophoresis system
PCR │ Choosing a thermocycler for teaching
PCR │ Performing an electrophoresis
Brochure│Our educational kits for teaching

The educational software PhorEasy® is available for free on the Jeulin digital platform.