Saccharomyces cerevisiae ADE2

The yeast of the saccharomyces cerevisiae ADE2 strain is delivered in lyophilized form in a glass vial. 
They must be rehydrated with sterile water, provided with the strain, before being cultured on a YPG minimal medium.
Attention, the water does not contain any nutrients. Once the yeast is rehydrated, it cannot be stored in suspension for more than 3 to 4 days. They need to be seeded on a Petri dish for preservation.  

Reactivation of yeast and culturing on a Petri dish:
- Add 2 to 3 ml of sterile water to the tube of lyophilized yeast to suspend the yeast.
- Vortex the yeast solution.
- Using an inoculation loop, spread the yeast on YPG medium (ref. 107122) on a Petri dish.
- Incubate for 2 to 4 days at 37°C to obtain yeast colonies.
At this stage, the yeast concentration is likely too high for clear visibility of colonies (CFU) on the Petri dish. To obtain well-separated colonies, suspend 2 colonies in a 2 ml tube of sterile water (approximately 1 colony of 1 mm for suspension in 1 mL of sterile water) and repeat the spreading protocol on a new Petri dish.
Another alternative: Prepare suspensions of known yeast concentrations at 10^3 cells/ml by counting them beforehand on a Malassez cell.

The product consists of:
- 1 glass vial containing 0.2 g of lyophilized yeast
- 1 tube of 3 mL of sterile water

- approximately 0.2 g of freeze-dried yeast in a glass bottle - 3mL of sterile water in tube