Impact of pollution on aquatic microflora Kit

This kit illustrates the consequences of the infiltration of phytosanitary products into the soil, here the copper sulfate used in organic agriculture known as "Bordeaux mixture", and then into natural waters (rivers, lakes). Principle: Students will test the toxic effects of a pesticide on two species of aquatic microfauna, a photosynthetic bacterium, and a microalgae. This experiment allows for the discussion of ecotoxicity concepts, lethal or non-lethal threshold concentrations that influence the reproduction rate or fitness of organisms. The consequences on organisms are visible to the naked eye. This observation can be complemented by microscopic examination. Experiment: During the experiment, students create a series of solutions with increasing pesticide concentrations. The solutions are added to the medium containing the aquatic organisms. Negative effects are observed within a few days. This kit contains living organisms. Plan to purchase the kit 15 days before the lab session (or more if there is multiplication of microorganisms in a suitable culture medium). Format: 6 tests (1 control + 6 concentrations) Duration of the experiment: 1 session of 60 min + 1 session to observe the results (effects of products over time + 24h, + 48h...) Storage duration: Maximum 1 week if the bottle is closed and kept away from light Storage conditions: Room temperature

The kit consists of:
- 1 bottle of cyanobacteria (50 ml)
- 1 bottle of scenedesmus (50 ml)
- 1 bottle of simulated phytosanitary solution (copper sulfate, quaternary amine) (20 ml)
- 56 sterile tubes
- 12 dropper pipettes
- cotton