Sickle Cell Anemia Kit of Analog Hemoglobins
Sku: 107842
Jeulin
The strong points
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Designed by Jeulin
€28.88
€34.66
Sickle cell disease is a genetic disorder characterized by the presence of abnormal hemoglobin S (HbS). The HbS hemoglobins are due to mutations in the gene coding for the β chain. Through hemoglobin electrophoresis, it is possible to identify the HbS molecular phenotypes from normal HbA hemoglobin.
This kit offers the ability to distinguish between the two types of hemoglobins by their difference in electrophoretic mobility, in order to determine the individual's genotype.
The electrophoresis is performed in agarose gel, compatible with any type of DNA electrophoresis. In this kit, the HbA and HbS hemoglobin solutions are simulated by 2 red-colored organic compounds that appear equivalent to blood samples. The kit contains the simulated hemoglobins and the reagents for migration (agarose, TAE buffer), all of which are completely safe.
Depending on the type of electrophoresis used, it is possible to perform:
- 10 gels (15 ml) with the compact phorEasy Jeulin or Minione systems --> 0.45 g of agarose / gel 3%
- 5 gels (40 ml) with the standard DNA/protein Jeulin tanks --> 1.2 g of agarose / gel 3%
Format: 40 tests (pairs) or 80 deposits
Migration time: 6 min
Storage: 12 months at room temperature
Separation of DNA fragments: 3% agarose gel (without gel green)
Storage: 12 months at room temperature
Separation of DNA fragments: 3% agarose gel (without gel green)
The kit consists of:
- Simulated HbS tube (350 μl)
- Simulated HbA tube (350 μl)
- Agarose (6 g)
- TAE buffer 10X (2 x 100 ml equivalent to 2 L of TAE 1X)
| Thématiques | Analyse de protéines - pigments |
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